Recipe

Annealing mix

10 mM Tris-Cl (pH 7.8)

1 mM EDTA

0.25 M KCl

0.83 nM radiolabeled oligonucleotide primer

This mix can be prepared as a 5X stock without primer and stored at -20ºC. Dilute with TE before use.

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  1. doi:10.1101/pdb.rec10894 Cold Spring Harb Protoc 2007: pdb.rec10894-
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