Recipe

Drosophila lysis buffer

Tris-Cl (50 mM, pH 7.5)

KCl (150 mM)

MgCl2 (5 mM)

Sucrose (0.25 M)

DTT (0.1 mM)

PMSF (1 mM)

Leupeptin (2 μg/ml)

Pepstatin (2 μg/ml)

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  1. doi:10.1101/pdb.rec10903 Cold Spring Harb Protoc 2007: pdb.rec10903-
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