Embryo buffer

Benzamidine (1 mM)

EGTA (20 mM)

MgCl₂ (15 mM)

EDTA (10 mM) may be used in place of 15 mM MgCl₂.

PMSF (phenylmethylsulfonyl fluoride) (2 mM)

Sodium β-glycerophosphate (80 mM, pH 7.5)

Sodium metabisulfate (1 mM)

Sodium orthovanadate (Na₃VO₄) (2 mM)

Tris-Cl (10 mM, pH 7.5)

Tween 20 (0.05%)