Table 1. Bacterial reporter strains
| Strain | Genotype | Comment/description |
| KJAG242A | ΔhisB463, Δ(gpt-proAB-arg-lac)XIII, zaj::Tn10 [F′ lacIq (Z321[-65] lacZYA*) KanR] |
Reporter strain in which the expression of the lacZ reporter gene is directed by a weak promoter bearing an upstream Zif268-binding site located at position -65a (in orientation 321b) |
| RP28 | ΔhisB463, Δ(gpt-proAB-arg-lac)XIII, zaj::Tn10 [F′ lacIq (λop[-62] lacZYA*) KanR] |
Reporter strain in which the expression of the lacZ reporter gene is directed by a weak promoter bearing an upstream λcI-binding site centered at position -62a |
| KDZif1ΔZ | araD(gpt-lac)5, rpsL (strR), ΔspoS3::cat (CamR) [F′ lacIq (Z321[-61] lacZYA*) KanR] |
Reporter strain in which the expression of the lacZ reporter gene is directed by a weak promoter bearing an upstream Zif268-binding site located at position -61a (in orientation 321b) |
| SII-LAM1 | araD(gpt-lac)5, rpsL (strR), [F′ lacIq (modified lacUV5 with λ operator lacZYA) KanR] |
Reporter strain in which the expression of the lacZ reporter gene is directed by a strong constitutive (modified lacUV5) promoter. Binding of a λcI DNA-binding domain to a λ operator positioned between the -35 and -10 elements of the promoter causes repression of lacZ reporter expression. |
| aIndicates position relative to the promoter transcription startpoint. | ||
| bIndicates orientation of asymmetric Zif268-binding site relative to the promoter. In orientation 321, the binding site is positioned such that the amino-terminal Zif268 finger is most proximal to the promoter. In orientation 123, the binding site is positioned such that the carboxy-terminal Zif268 finger is most proximal to the promoter. | ||
