Table

Table 1. Choice of matrix for MALDI-MS analysis of different analytes

Matrixa Peptide mappingb Small proteins Large proteins Glyco peptides Glyco proteins
HCCA 1c 3 - 2 -
SA - 1 1 3 2
DHB 2 2 1 1 1
THAP 2 - - - -
DHAP/DAHC 2 - - -
a(HCCA) α-cyano-4-hydroxy-cinnamic acid; (SA) sinapinic acid; (DHB) 2,5-dihyroxybenzoic acid; (THAP) 2,4,6-trihydroxyacetophenone; (DHAP) 2,6-dihydroxyacetophonone; (DAHC) diammonium hydrogen citrate.
bThe choice of matrix can considerably influence the sequence coverage in peptide mapping. For example, peptide mapping data obtained with the matrix THAP can complement those obtained with DHB and HCCA (Kussman and Roepstorff 2000).
cThe choice of matrix must be adapted to the physical properties of the analyte. First choice is denoted by 1, second choice by 2, and third choice by 3.
Portion adapted from Kussmann and Roepstorff (2000). Reproduced with permission © 2000 Humana Press.
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  1. doi:10.1101/pdb.tab194679 Cold Spring Harb Protoc 2007: pdb.tab194679-

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