Table 2. Bacterial two-hybrid reporter strains with Zif268-binding sites placed at various distances and orientations relative to the transcription startpoint
| Strain designationa | Location of Zif268-binding site (relative to transcription startpoint) |
Orientation of Zif268-binding site |
| KJAG275A | -46 | 321 |
| KJAG293A | -48 | 321 |
| KJAG324A | -50 | 321 |
| CC2-A | -61 | 321 |
| KJAG232A | -63 | 321 |
| KJAG338B | -67 | 321 |
| KJAG311A | -69 | 321 |
| KJAG319B | -71 | 321 |
| KJAG290A | -42 | 123 |
| KJAG267A | -44 | 123 |
| KJAG274A | -46 | 123 |
| KJAG294A | -48 | 123 |
| KJAG326A | -50 | 123 |
| KJAG386A | -55 | 123 |
| KJAG383A | -57 | 123 |
| KJAG378A | -59 | 123 |
| CC1-A | -61 | 123 |
| KJAG237A | -63 | 123 |
| KJAG243A | -65 | 123 |
| KJAG336A | -67 | 123 |
| KJAG300B | -69 | 123 |
| KJAG302A | -71 | 123 |
| aAll strains listed have the genotype ΔhisB463, Δ(gpt-proAB-arg-lac)XIII, zaj::Tn10 [F′ lacIq (modified lacZYA) KanR] but differ in the location and orientation of the Zif268-binding site relative to the promoter that controls lacZ expression on the F′. | ||
| bIndicates orientation of asymmetric Zif268-binding site relative to the promoter. In orientation 321, the binding site is positioned such that the amino-terminal Zif268 finger is most proximal to the promoter. In orientation 123, the binding site is positioned such that the carboxy-terminal Zif268 finger is most proximal to the promoter. | ||
