Table 3. Expression plasmids for DBD hybrid proteins
| Plasmid name | Antibiotic marker | Plasmid origin | Comment/description |
| pBR-UV5-X-Zif268 | Ampicillin | ColEI | Phagemid to clone protein X as a fusion amino-terminal to Zif268 (residues 327-421); expression driven by the IPTG-inducible lacUV5 promoter; has f1 ori of replication |
| pACYC-Zif268-X | Tetracycline | p15A | Plasmid to clone protein X as a fusion carboxyl-terminal to Zif268 (residues 327-421); expression driven by the IPTG-inducible lacUV5 promoter |
| pACYC-X-Zif268 | Tetracycline | p15A | Plasmid to clone protein X as a fusion amino-terminal to Zif268 (residues 327-421); expression driven by the IPTG-inducible lacUV5 promoter |
| pBT | Chloramphenicol | p15A | Plasmid to clone protein X as a fusion to the carboxyl terminus of the λcI protein; expression driven by the IPTG-inducible lacUV5 promoter |
| pBR-UV5-λcI-X | Ampicillin | ColEI | Phagemid to clone protein X as a fusion to the carboxyl terminus of the λcI protein; expression driven by the IPTG-inducible lacUV5 promoter; has f1 ori of replication |
| pACλcI-Gal11P (positive control) |
Chloramphenicol | p15A | Control plasmid that expresses λcI-Gal11P fusion protein driven by the IPTG-inducible lacUV5 promoter |
| pBT-LGF (positive control) |
Chloramphenicol | p15A | Control plasmid that expresses λcI-Gal4 fusion protein driven by the IPTG-inducible lacUV5 promoter |
| Sequences for all plasmids are available on the Joung lab Web site at www.zincfingers.org. | |||
