Table 4. Expression vectors for E. coli RNAP subunit hybrid proteins
| Plasmid name | Antibiotic marker | Plasmid origin | Comment/description |
| pACYC-α-Y | Chloramphenicol | p15A | Plasmid to clone protein Y carboxyl-terminal to the E. coli RNA polymerase α-subunit (residues 1-248); expression driven by the IPTG-inducible tandem lpp/lacUV5 promoter |
| pBR-UV5-Y-αLP31.1 | Ampicillin | ColEI | Phagemid to clone protein Y carboxyl-terminal to the E. coli RNA polymerase α-subunit (residues 1-248); expression driven by the IPTG-inducible lacUV5 promoter; has f1 ori of replication |
| pBR-UV5-ω-Y | Ampicillin | ColEI | Plasmid to clone protein Y carboxyl-terminal to the E. coli RNA polymerase ω-subunit; expression driven by the IPTG-inducible lacUV5 promoter |
| pBR-UV5-Y-ω | Ampicillin | ColEI | Plasmid to clone protein Y N-terminal to the E. coli RNA polymerase ω-subunit; expression driven by the IPTG-inducible lacUV5 promoter |
| pTRG | Tetracycline | ColEI | Plasmid to clone protein Y carboxyl-terminal to the E. coli RNA polymerase α-subunit (residues 1-248); expression driven by the IPTG-inducible tandem lpp/lacUV5 promoter |
| pBR-αGal4 (positive control) |
Ampicillin | ColEI | Control plasmid that expresses an α-Gal4 fusion protein driven by the IPTG-inducible tandem lpp/lacUV5 promoter |
| pTRG-Gal11P (positive control) |
Tetracycline | ColEI | Control plasmid that expresses an α-Gal11P fusion protein driven by the IPTG-inducible tandem lpp/lacUV5 promoter |
| Sequences for all plasmids are available on the Joung lab Web site at www.zincfingers.org. | |||
