Staining Frozen Mouse Embryo Sections for β-Galactosidase (lacZ) Activity
This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems,” Chapter 16, in Manipulating the Mouse Embryo, 3rd edition (eds. Nagy et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.INTRODUCTION
Frozen mouse embryo sections are stained for β-galactosidase (lacZ) activity using X-gal followed by counterstaining with Orange G.
