Recipe

2X Laemmli sample buffer with β-mercaptoethanol

100 mM Tris-Cl (pH 6.8)

2% SDS

20% glycerol

4% β-mercaptoethanol (added fresh)

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  1. doi:10.1101/pdb.rec10767 Cold Spring Harb Protoc 2007: pdb.rec10767-
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