Kinase reaction buffer B
100 μM ATP analog
1-5 μg of a known substrate/reaction
10 μCi/reaction [γ-32P]ATP analog (required only if a phospho-specific antibody to the substrate is not available). Preparation of [γ-32P]ATP analog is described in Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Generation of [γ-32P]ATP Analog from ADP Analog.
