PBS (10X) without potassium salt
| Reagent | Amount to add (for 1L) | Final (10X) concentration |
|---|---|---|
| NaCl | 75.97 g | 1.3 M |
| Na2HPO4•2H2O | 12.46 g | 70 mM |
| NaH2PO4•2H2O | 4.8 g | 30 mM |
| Combine all components in <1 L of H2O and stir to dissolve. Adjust pH to 7.2 and bring to final volume of 1 L with H2O. Sterilize by autoclaving. | ||
