Recipe

PLP

Mix the following to create a buffered lysine solution:

Lysine 0.36 g
NaH2PO4 (0.1 M, pH 7.2) (cold) 7.5 ml
Na2HPO4 (0.1 M, pH 7.2) (cold) 2.5 ml
H2O (cold) 10 ml

Immediately before use, add 15 ml of the buffered lysine solution to the following:

Formaldehyde (8%, freshly prepared) 5 ml
Sodium m-periodate (NaIO4) 50 mg

The final composition of PLP is 0.075 M lysine, 0.37 M sodium phosphate (pH 7.2), 2% formaldehyde, and 0.01 M NaIO4. It is not necessary to pH the final solution. PLP is a gentle fix and should be used on ice.

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  1. doi:10.1101/pdb.rec10921 Cold Spring Harb Protoc 2007: pdb.rec10921-
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