Recipe

Detergent rinse

MgCl2 (2 mM)

Nonidet P-40 (NP-40; Sigma N6507) (0.02%)

Phosphate buffer (0.1 M, pH 7.3), prepared using phosphate buffer tablets (e.g., BDH)

Sodium deoxycholate (0.01%)

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  1. doi:10.1101/pdb.rec10945 Cold Spring Harb Protoc 2007: pdb.rec10945-
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