Fixative for staining intact embryos
EGTA (5 mM)
This should be from a stock of 0.1 M EGTA at pH 8.0.
MgCl2 (2 mM)
Phosphate buffer (0.1 M, pH 7.3), prepared using phosphate buffer tablets (e.g., BDH)
EGTA (5 mM)
This should be from a stock of 0.1 M EGTA at pH 8.0.
MgCl2 (2 mM)
Phosphate buffer (0.1 M, pH 7.3), prepared using phosphate buffer tablets (e.g., BDH)