Recipe

Fixative for staining frozen sections

EGTA (5 mM)

MgCl2 (2 mM)

2% paraformaldehyde (2%, w/v) in PIPES buffer (0.1 M, pH 6.9) (Sigma P9291)

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  1. doi:10.1101/pdb.rec10949 Cold Spring Harb Protoc 2007: pdb.rec10949-
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