Recipe

PBS with protease inhibitors

PBS for GST fusion protein preparation

Just prior to use, add protease inhibitors, e.g.,

2 μg/ml aprotinin

1 μg/ml leupeptin

25 μg/ml PMSF

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  1. doi:10.1101/pdb.rec10976 Cold Spring Harb Protoc 2007: pdb.rec10976-
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