Table 1. Simple cross-linking screen
| Reaction | Molar excess cross-linkera (mole cross-linker/mole protein) |
pH (HEPES buffer) | Time |
|---|---|---|---|
| 1 | 102 | 7.0 | 5 min, 20 min |
| 2 | 102 | 7.5 | 5 min, 20 min |
| 3 | 102 | 8.0 | 5 min, 20 min |
| 4 | 103 | 7.0 | 5 min, 20 min |
| 5 | 103 | 7.5 | 5 min, 20 min |
| 6 | 103 | 8.0 | 5 min, 20 min |
| 7 | 104 | 7.0 | 5 min, 20 min |
| 8 | 104 | 7.5 | 5 min, 20 min |
| 9 | 104 | 8.0 | 5 min, 20 min |
| aFor complex mixtures, add 10 μmol of cross-linker per milligram of protein. | |||
