Protocol

Xenopus laevis In Vitro Fertilization and Natural Mating Methods

This protocol was adapted from “Obtaining Embryos,” Chapter 5, in Early Development of Xenopus laevis by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.

INTRODUCTION

This protocol describes in vitro fertilization and natural mating methods for Xenopus laevis.

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