Recipe

Hybridization master mix

8 g dextran sulfate (Amersham)

SSC, diluted to 2X

Dissolve 8 g dextran sulfate in 40 ml of 2X SSC, vortex, and filter using a 0.45-μm filter. Store in aliquots at −20°C.

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  1. doi:10.1101/pdb.rec10964 Cold Spring Harb Protoc 2007: pdb.rec10964-
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