Recipe

2X gel-loading buffer for HPLC/SDS-PAGE

125 mM Tris-Cl (pH 6.8)

20% glycerol

4% SDS

100 mM DTT

0.04% (w/v) bromophenol blue

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  1. doi:10.1101/pdb.rec10985 Cold Spring Harb Protoc 2007: pdb.rec10985-
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