Recipe

Rehydration buffer A

0.5 M Tris-Cl (pH 8.3)

8 M urea

0.5% Triton X-100

2 mM dithiothreitol (DTT)

0.2% Ampholine (Amersham Biosciences)

Choose the Ampholine set with the appropriate pH range for the proteins to be separated.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec10992 Cold Spring Harb Protoc 2007: pdb.rec10992-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue