Recipe

Rehydration buffer A

0.5 M Tris-Cl (pH 8.3)

8 M urea

0.5% Triton X-100

2 mM dithiothreitol (DTT)

0.2% Ampholine (Amersham Biosciences)

Choose the Ampholine set with the appropriate pH range for the proteins to be separated.

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  1. doi:10.1101/pdb.rec10992 Cold Spring Harb Protoc 2007: pdb.rec10992-
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