Table 1. The combined use of different fluorochromes for FISH in 3D preserved cultured mammalian cells
| Labeling | Fluorochrome | Detection |
|---|---|---|
| --- | DAPI (blue) | Staining with 0.05 μg/ml DAPI in 4XSSC/0.2% Tween for 5 minutes |
| bio-dUTP | FITC (green) | First layer: avidin conjugated to Alexa 488 Second layer: goat-anti-avidin conjugated to FITC |
| dnp-dUTP | Alexa-514 (yellow) | First layer: rabbit-anti-dnp Second layer: goat-anti-rabbit conjugated to Alexa 514 |
| Cy3-dUTP | Cy3 (orange) | Cy3-dUTP |
| Texas Red-dUTP | Texas Red (red) | Texas Red-dUTP |
| dig-dUTP | Cy5 (far red) | First layer: mouse-anti-dig conjugated to Cy5 Second layer: goat-anti-mouse conjugated to Cy5 |
