Protocol

Preparation of Zebrafish Embryos for Transmission Electron Microscopy

  1. Reinhard W. Köster
  1. GSF-National Research Center for Environment and Health, Institute of Developmental Genetics, 85764 München-Neuherberg, Germany
  1. ↵1Corresponding author (sandra.rieger{at}gsf.de)

INTRODUCTION

This protocol describes a procedure for the fixation and embedding of zebrafish embryos at organogenesis stages (48-72 hours post-fertilization [hpf]) for transmission electron microscopy (TEM). The lengths of individual steps may be adjusted according to the developmental stage of the specimen.

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  1. doi:10.1101/pdb.prot4772 Cold Spring Harb Protoc 2007: pdb.prot4772-
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