Recipe

RNA FISH hybridization mix

1 part 20X SSC (pH 7.4)

2 parts 10 mg/ml BSA (New England Biolabs)

2 parts 50% (w/v) dextran sulfate solution

5 parts formamide (Fluka)

Mix on a shaker for 30 minutes at room temperature. Store at −20°C.

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  1. doi:10.1101/pdb.rec11014 Cold Spring Harb Protoc 2007: pdb.rec11014-
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