Recipe

Sample resuspension buffer B

1.0% β-mercaptoethanol

Add β-mercaptoethanol fresh just before use.

5 mM EDTA

200 mM NaCl

100 mM Tris-Cl (pH 8.0)

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  1. doi:10.1101/pdb.rec11032 Cold Spring Harb Protoc 2007: pdb.rec11032-
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