Recipe

Dialysis-lysis buffer

1 mM Tris-Cl (pH 7.5)

0.2 mM EDTA

0.2 mM PMSF

Protease inhibitors, and in particular PMSF, are rather unstable in aqueous solutions. Therefore, PMSF should be added as late as possible, just before use.

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  1. doi:10.1101/pdb.rec11035 Cold Spring Harb Protoc 2007: pdb.rec11035-
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