Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes
- Rosa A. Uribe1,2 and
- Jeffrey M. Gross1,2,3,4
- 1 Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, Texas 78712, USA
- 2 Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
- 3 Institute for Neuroscience, The University of Texas at Austin, Austin, Texas 78712, USA
- ↵4Corresponding author (jmgross{at}mail.utexas.edu)
INTRODUCTION
This protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues.
