Recipe

Regeneration buffer

100 mM glycine (pH 2.8)

The regeneration buffer must be sterile, and it does not last long. Autoclave before use, and store at 4ºC, where it will last 1 week.

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  1. doi:10.1101/pdb.rec10956 Cold Spring Harb Protoc 2007: pdb.rec10956-
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