Recipe

2X Cell-freezing medium

40% (v/v/v) Dulbecco’s modified Eagle’s medium (DMEM; Gibco/Invitrogen 11960-044)

40% (v/v/v) Fetal bovine serum (FBS; Hyclone SH30070.03)

20% (v/v/v) Dimethylsulfoxide (DMSO; Sigma-Aldrich D2650)

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  1. doi:10.1101/pdb.rec11007 Cold Spring Harb Protoc 2007: pdb.rec11007-
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