Staining Mouse Embryos for Alkaline Phosphatase Activity
This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems,” Chapter 16, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.Abstract
This protocol describes a technique that is used to visualize human placental alkaline phosphatase (AP) activity in transgenic mice. AP activity staining is localized to the cell surface. Endogenous AP activity is eliminated by heat inactivation that spares the heat-stable human placental AP activity.
