Recipe

Danieau solution (300%)

Reagent Amount to add
2.9 M NaCl 60 mL
70 mM KCl 30 mL
40 mM MgSO4 • 7H2O 30 mL
60 mM Ca(NO3)2 30 mL
0.5 M HEPES (pH 7.2) 30 mL
H2O to 1000 mL

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  1. doi:10.1101/pdb.rec11095 Cold Spring Harb Protoc 2007: pdb.rec11095-
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