Drosophila Cell Culture and Transformation
Adapted from “Drosophila Cell Culture and Transformation,” Chapter 20, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
Permanent Drosophila cell lines derived from mixed embryonic tissues including the most commonly used lines, S2 and Kc, have been available for ~30 yr. More recently, lines derived from specific tissues, imaginal discs, and the larval central nervous system have come into use. Although cultured cells were originally used by Drosophilists mainly as convenient sources of DNA or carrier RNA, that situation has changed, as an armamentarium of techniques for using the cells has slowly but steadily evolved. Most investigators use Drosophila cell lines as hosts for transformation experiments. The goal may be to characterize a promoter, to investigate the role of a transcription factor, to overexpress a polypeptide, or to do something more novel. This article provides an organized collection of pointers to published protocols.
