Genotyping by Allele-Specific Amplification (KASPar)
- Corresponding author (ecuppen{at}niob.knaw.nl)
INTRODUCTION
This protocol describes the use of allele-specific amplification followed by fluorescence detection (KASPar) for genotyping. The method is compatible with any real-time PCR setup and has the advantage that no specifically labeled oligonucleotides are needed.
