Protocol

Genotyping by Dideoxy Resequencing

  1. Edwin Cuppen
  1. Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands
  1. Corresponding author (ecuppen{at}niob.knaw.nl)

INTRODUCTION

This protocol describes dideoxy resequencing of PCR fragments, which is a quick and flexible genotyping technique. This strategy is used to identify and confirm the presence of heterozygous single-nucleotide polymorphisms (SNPs) within a genomic region of interest.

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  1. doi:10.1101/pdb.prot4842 Cold Spring Harb Protoc 2007: pdb.prot4842-
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