Genotyping by Oligonucleotide Ligation Assay (OLA)
- Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA
- Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA
- Corresponding author (sjmac{at}ku.edu)
INTRODUCTION
This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes.
