Recipe

In-gel protease solution

Trypsin (0.05 μg/μL)

Use sequencing grade, modified trypsin (e.g., from Promega).

NH4HCO3 (10 mM, pH 7.8)

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  1. doi:10.1101/pdb.rec10762 Cold Spring Harb Protoc 2007: pdb.rec10762-
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