Drosophila fruit juice egg plates
This procedure makes 1 L of solution and yields ~170 juice plates.
MATERIALS
Reagents
30 g agar
10 mL ethanol
300 mL fruit juice concentrate (grape or apple)
700 mL H2O
Equipment
Autoclave
Petri dish lids (plastic, 10 × 35 mm)
METHOD
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1. Add the agar to the H2O. Autoclave for 40 min.
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2. While autoclaving, spread the tops of the Petri dishes face up on a series of trays.
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3. Dissolve the methyl paraben in the ethanol.
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4. Add the methyl paraben solution to the juice concentrate.
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5. Quickly and thoroughly mix the concentrate into the autoclaved agar solution.
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6. Immediately begin dispensing media into dishes.
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7. Once the juice plates have cooled and hardened, store them at 4°C.
