Immunohistochemistry of Embryo Sections
This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems,” Chapter 16, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.INTRODUCTION
This protocol describes how to localize an antigen in cells and tissues using embryo sections attached to glass slides. The method outlined here uses alkaline-phosphatase-coupled secondary antibody; horseradish-peroxidase-coupled secondary antibody can be used as an alternative.
