Preparation and Loading of Gelatin Nanoparticles
This protocol was adapted from “Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles,” Chapter 52, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2007.INTRODUCTION
This protocol describes the synthesis of nanoparticles using the biopolymer gelatin and its derivatives, the loading of DNA to the nanoparticles by encapsulation and adsorption, and the estimation of entrapment efficiency. The steps involved in the preparation of nanoparticles by desolvation using ethanol are presented, as are the precautions to be taken during the encapsulation or adsorption of the payload.
