Cell Transfection and Analysis Using DNA-Loaded Gelatin Nanoparticles
This protocol was adapted from “Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles,” Chapter 52, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2007.INTRODUCTION
This protocol describes cell-culture techniques for transfection using DNA-loaded gelatin nanoparticles. Also included are methods for qualitative analysis of transfection with fluorescence microscopy and quantitative analysis using a fluorescence-activated cell sorter (FACS).
