Recipe

Krebs-Ringer superfusion solution

125 mM NaCl

2.5 mM KCl

1.25 mM NaH2PO4

2 mM CaCl2

1 mM MgCl2

25 mM NaHCO3

25 mM glucose

Bubble in carbogen (95% O2 and 5% CO2) for 30 min to reach a pH of 7.4.

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  1. doi:10.1101/pdb.rec11150 Cold Spring Harb Protoc 2008: pdb.rec11150-
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