Protocol

Isolation of Pristionchus Nematodes from Beetles

  1. Ralf J. Sommer1
  1. Max-Planck Institute for Developmental Biology, Department of Evolutionary Biology, D-72076 Tübingen, Germany
  1. 1Corresponding author (ralf.sommer{at}tuebingen.mpg.de)

INTRODUCTION

In this procedure, nematodes disembark from a beetle carcass and feed on Escherichia coli OP50. The nematodes are then monitored for a few days and identified using simple morphological characteristics. This method is rapid, easy, and biased for Pristionchus species.

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