Enzyme solution for neurosphere assay
200 μL papain (Roche Applied Science)
-
1. Combine above reagents and incubate for 15 min in a 37°C water bath to dissolve.
-
2. Mix and adjust to pH ~7.4 with 0.1 N NaOH (this usually requires ~3 drops of 0.1 N NaOH). Monitor the pH by the color of the solution: Pink is too basic and yellow is too acidic.
-
3. Filter through a 0.2-μm syringe filter.
-
4. Warm the solution in a water bath at 37°C before use. Prepare immediately before use.
