Recipe

NEP basal medium

48.5 mL neurobasal medium (PSG)

1 mL B-27 supplement (50X; GIBCO)

0.5 mL N-2 supplement (100X; GIBCO)

Combine above reagents in a 50-mL conical tube. Mix well.

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  1. doi:10.1101/pdb.rec11502 Cold Spring Harb Protoc 2008: pdb.rec11502-
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