Recipe

NEP complete medium

10 mL NEP basal medium

10 μL epidermal growth factor solution (EGF) (100 ng/μL in 0.1% BSA)

10 μL basic fibroblast growth factor solution (bFGF) (10 ng/μL in 0.1% BSA)

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  1. doi:10.1101/pdb.rec11506 Cold Spring Harb Protoc 2008: pdb.rec11506-
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