Temporary medium
| Reagent | Amount to add (for 1 L) | Final concentration |
|---|---|---|
| Agar | 8 g | 0.8% |
| 500X Gamborg’s B5 vitamins stock solution | 2 mL | 1X |
| Glucose | 30 g | 3% |
| MS (Murashige and Skoog) basal salt mixture | 4.3 g | 0.43% |
| Prepare the medium in H2O. Adjust the pH to 5.5 using KOH, autoclave the solution, and pour 30 mL into each Petri dish (100 mm diameter × 20 mm high). Store the plates for up to 1 mo at 4°C. | ||
