Recipe

AP developing buffer

Reagent Quantity (for 10 mL) Final concentration
5-Bromo-4-chloro-3-indolyl-phosphate (BCIP) (50 mg/mL) 33 μL 0.165 mg/mL
4-Nitro blue tetrazolium chloride (NBT) (100 mg/mL) 33 μL 0.33 mg/mL
Alkaline phosphatase (AP) ctenophore buffer (with MgCl2) 10 mL
Prepare fresh. Store at room temperature.

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  1. doi:10.1101/pdb.rec11523 Cold Spring Harb Protoc 2008: pdb.rec11523-
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