| Reagent |
Quantity (for 500 mL) |
Final concentration |
| Maleic acid (1 M, pH 7.5)
|
50 mL |
0.1 M |
| NaCl (5 M) |
15 mL |
0.15 M |
| H2O
|
to 500 mL |
|
| Adjust the pH to 7.5 with NaOH. Sterilize by autoclaving for 20 min at 15 psi and store at room temperature. Buffer will keep
for 6-12 mo.
|